|
KMID : 0903520080510010065
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2008 Volume.51 No. 1 p.65 ~ p.69
|
|
|
Development of Biologically Active Compounds from Edible Plant Sources XXII. Isolation of Indoles from the Roots of Brassica campestris ssp rapa and their hACAT Inhibitory Activity
|
|
Bang Myun-Ho
Lee Dae-Young
Oh Young-Jun
Han Min-Woo
Yang Hye-Joung
Cheng Hae-Gon
Jeong Tae-Sook
Lee Kyung-Tae
Choi Myoung-Sook
Baek Nam-In
|
|
|
Abstract
|
|
|
|
The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatography. From the results of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as caulilexin C (1), indoleacetonitrile (2) and arvelexin (3). The arvelexin (3) has been isolated from this plant for the first time. Compounds 1, 2 and 3 showed inhibitory activity on human Acyl CoA: cholesterol transferase 1 (hACAT1) by 54.6±6.0%, 69.2±4.7% and 68.6±3.7%, and on human Acyl CoA: cholesterol transferase 2 (hACAT2) by 4.8±13.4%, 45.6±4.8% and 39.5±4.3%, respectively, at 100 μg/ml.
|
|
|
KEYWORD
|
|
|
arvelexin, Brassica campestris ssp rapa, caulilexin C, hACAT, indoleacetonitrile
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|